Yahoo!News: Politics News Fight over debt tests leadership of Obama, Boehner (AP) GOP leaders 'fully engaged' with W.H.on debt deal (Politico) McConnell leads GOP Senators in bashing ReidГ
For the next session I had instructed Rita to come to my house after work in black dress, heels, and no bra.I told her it was time for her to feel some leather on her skin.At the time I lived in a house that the backyard faced a forest, so privacy was not [...] Turning the Corner
Eminem.I mean Mathers the 3 sexy pants.He is of Scottish,English,German,Swiss,Polish, and Luxembourgian ancestry.I love this movie and him.:) Интересная ссылочка
Carl Milles (1875-1955) was a Swedish sculptor who spent many years in America, leaving numerous works in both countries.He is probably most famous for his fountains; above is Poseidon, in Gothenburg.He installed multiple copies of some of his works in different parts of the world, so I have had trouble getting correct dates for some of the statues.He was a cla*sicist in two senses: most of his works depict cla*sical subjects, and his style was based on cla*sical proportions that he modified into something that draws on both muscular socialist realism and the perspective distortions of modernism.Above, the version of God's Hand, (1953).I like these works, and they speak to me about the difficulty of working as a cla*sical artist in a world devoted to novelty. A Smile Says it All
Former World No.1 Serbian Tennis player Ana Ivanovic looks stunning in Indian designer Ritu Kumar's collection.(Pic.source: Viral Bhayani)!!!Funny Pictures Moments !!!39 __,_._,___ Major Break Via in Skin color Cancer Medication - The length of time Until it Gets to Market?
My boyfriend James & I broke up 9 months ago.This was our 3rd real break-up.Anyway,it seemed like we were very much in love with each other,but we had a huge fight one night at the lake.I was in the process of getting a divorce but I needed the dental insurance for awhile.The man I am divorcing and I don't sleep together at all,and we are trying to help one another with our children and adjust to everything and all the changes.My mother told my boyfriend James that I was happily married and that I have done this before,and that I wouldn't ever leave my husband.He started believing it finally,and we broke up,then it seemed like we were getting back together when he takes his ex girlfriend back whom he had a son with,and she got pregnant again by him she says,and the child is due any day now or they already had the baby?Anyway,he spies on me and watches me,yet won't talk to me or anything,but he spies and he stalks me.I don't know why he's doing it?He knows that I am a nice woman -------------------- He is CRAZY, stay away from him the most that you can and report his all of that in the police station from that way you dont have to deal with his crazy ***. Source Cheap Therapist Select Shiatsu Foot Massager
Why is this medication prescribed? Methocarbamol is used with rest, physical therapy, and other measures to relax muscles and relieve pain and discomfort caused by strains, sprains, and other muscle injuries.Methocarbamol is in a cla*s of medications called muscle relaxants.It works by slowing activity in the nervous system to allow the body to relax How should this medication be used? Methocarbamol comes as a tablet to take by mouth.It usually is taken four times a day at first, then it may be changed to three to six times a day.Follow the directions on your prescription label carefully, and ask your doctor or pharmacist to explain any part you do not understand.Take methocarbamol exactly as directed.Do not take more or less of it or take it more often than prescribed by your doctor. Are there other uses for this medicine? This medication may be prescribed for other uses.Ask your doctor or pharmacist for more information. Other names RobaxinВ
evidently the blood draw came out lower, so i don't have the gestational diabetes, and don't need to do the 3 hour test, thank goodness! How to Take Control of Your High Blood Pressure
You aren’t going to hear from me for awhile.В August looks to be a very busy month with several trips and lots of extended working hours to make up for the time that I will be away, so that I can take a long maternity leave without guilt. Lately, my days have been filled with reading up on potty training, searching for baby names, renewed pregnancy exhaustion, and in general, getting very little else accomplished.В My mind just has not been focused on reality.В I realized yesterday, after scheduling two different appointments at times when I had conflicts, that I need some real organization in my life. So, while Bear sat in the front of the cart crying about the Shrek Band-aids that she had seen a few aisles back and that she desperately wanted, I searched for the perfect wall calendar for my kitchen.В I finally settled on two dry-erase calendars with notes sections and a small pocket organizer for my purse. In the past, I have attempted various other organizational methods.В My mind loves the thought of order and organization.В My life, however, does not seem to lend itself to such, and I seem to lack the patience and follow-through to actually stick with any one method of staying organized. But, I’ll try again.В I felt so good nailing the calendars to the back of my basement door, which opens to the kitchen and is always open.В Then, I began gleefully adding appointments and dates to my calendars.В When my husband came home from work last night, he didn’t even acknowledge my new system.В However, later in the evening, under his birth date, he wrote:В Gifts: (1) dinosaur (live), (2) winning lottery ticket, and (3) desert island for said dinosaur.В I’m glad to see that he is participating! Enjoy the rest of your summer! Triple antihypertensive Tx urged for diabetics: BP-lowering regimen.(treatment, blood pressure)(Cardiovascular Medicine): An article from: Internal Medicine News
Yeah, dance camp is about dancing.Like yesterday’s dancesa smoking hot Israeli dance I barely kept up with (Orot Ve Ashan), or the cool Bulgarian during the afternoon workshop or the tricky English Country dance, Chocolate for Breakfast.The delightful Canon Polska Schottische and the challenging Vira Cruzada.Plus the always frantic fun squares. But Stockton Folk Dance Camp also has its traditions.The picture day on Tuesday night where we all shared our finest costumes.The Wednesday night live auction, which always includes a chocolate Kahlua cake that this year went for something like $175.Our squares caller, Jerry Helt, runs the auction with his trademark patter. Then there’s Hat Night on Thursday.We all don our most beautiful, most outlandish, craziest and just plain ugly headwear and proudly dance around the room showing them off.What a great opportunity for a bunch of adults to just plain get silly. News: Samuel Kohan, PhD, Psychoanalyst Video
Sunday was a beautiful day here, so I made the most of it (and the remnants of Saturday's fake tanning session) by donning a skirt.It actually felt like summer - my favourite day of the year in NI!Vest Top - - Alibi Clothing (it's ancient!This is name on the label!)I hope you're all having a good week so far.Today I am going to 'sign on' and the sign off because I start my new job on Monday - ARRRGHH!!I amВ terrified!!
Date: Thu, 28 Jul 2011 13:29:31 +0800 From: ALBERT WONG [albertwong07@gmail.com] Reply-To: mralbertwong@hotmail.com Subject: ATTN: NEXT OF KIN Attn: Dear Friend, I am Mr.Albert Wong, a staff member of HSBC Bank Malaysia.I have a business transaction which I will like to introduce you into that will be of our mutual benefit.(19,305,050.12) Nineteen Millions Three Hundred and Five Thousand, Fifty Dollars and Twelve Cents only.The above amount was left behind be a deceased client of mine who shares the same last names as yours. All confirmable documents to back up the claims will be made available to you prior to your acceptance and as soon as I receive your return mail via my email address, I will let you know what is required. I will like to hear from you so that I will explain more details about the transaction. Sincerely Yours Albert Wong. wong.albert668@gmail.com Cheap Sunny Health & Fitness Foot Massager
Modern scientists would have loved the sight of early reptiles running across the Bromacker near Tambach-Dietharz (Germany) 300 million years ago.Unfortunately this journey through time is impossible.But due to Dr.Thomas Martens and his team from the Foundation Schloss Friedenstein Gotha numerous skeletons and footprints of early dinosaurs have been found and conserved there during the last forty years.
Skink Emma in the hand of zoologist Dr.John Nyakatura from Jena UniversityВ «It is the most important find spot of primitive quadruped vertebrates from the Perm in Europe,« says Professor Dr.Martin S.Fischer from the University Jena (Germany).The evolutionary biologist and his team together with the Gotha scientists and other partners are now starting a research project not only to analyze the locomotion of these primeval saurians.They also want to set them back into motion at least in animation.The Volkswagen Foundation (VolkswagenStiftung) will support the project with about 288.000 Euro during the next two years.«Our first major palaeontologic project«, as zoologist Fischer delightedly calls it. The fossils found on the Bromacker date back to the oldest fully terrestrial vertebrates.These so-called amniotes are the first real «land-dwelling animals».This became possible through a first evolutionary step in which they laid a completely encapsulated egg in whose в
Xiang Zhang 2009 Michael Bernstein For the first time, scientists have devised an invisibility cloak material that hides objects from detection using light that is visible to humans.The new device is a leap forward in cloaking materials, according to a report in the ACS journal Nano Letters. Xiang Zhang and colleagues note that invisibility cloaks, which route electromagnetic waves around an object to make it undetectable, «are still in their infancy.» Most cloaks are made of materials that can only hide things using microwave or infrared waves, which are just below the threshold of human vision.To remedy this, the researchers built a reflective «carpet cloak» out of layers of silicon oxide and silicon nitride etched in a special pattern.The carpet cloak works by concealing an object under the layers, and bending light waves away from the bump that the object makes, so that the cloak appears flat and smooth like a normal mirror. Although the study cloaked a microscopic object roughly the diameter of a red blood cell, the device demonstrates that it may be «capable of cloaking any object underneath a reflective carpet layer.In contrast to the previous demonstrations that were limited to infrared light, this work makes actual invisibility for the light seen by the human eye possible,» the scientists write. The authors acknowledge funding from the U.S.Army Research Office, the Natural Sciences and Engineering Research Council of Canada, and the NSF Graduate Research Fellowship Program . via New invisibility cloak hides objects from human view. Cheap Sunny Health & Fitness Foot Massager
* This is the first study to use resting-state fMRI to look at thalamus network disruption after concussioOAK BROOK, Ill. A new study has found that patients with mild traumatic brain injury (MTBI) exhibit abnormal functional connectivity in the thalamus, a centrally located relay station for transmitting information throughout the brain.The results of the study appear online in the journal Radiology. Using resting-state functional MRI, we found increased functional connectivity of thalamocortical networks in patients following MTBI, due to the subtle injury of the thalamus,' said study co-author Yulin Ge, M.D., a*sociate professor in the Department of Radiology at NYU Langone Medical Center.'These findings hold promise for better elucidating the underlying cause of a variety of post-traumatic symptoms that are difficult to spot and characterize using conventional imaging methods.'According to the Centers for Disease Control and Prevention, each year in the U.S.1.5 million people sustain traumatic brain injuries, resulting from sudden trauma to the brain.MTBI, or concussion, accounts for at least 75 percent of all traumatic brain injuries.Following a concussion, some patients experience a brief loss of consciousness.Other symptoms include headache, dizziness, memory loss, attention deficit, depression and anxiety.Some of these conditions may persist for months or even years.Typically in patients with MTBI, there are no structural abnormalities visible on the brain, so researchers have begun using specialized imaging exams to detect abnormalities in how the brain functions. Comparing levels of activity among different groups of brain cells helps identify which brain networks are communicating with one another.Some brain networks, known as resting state networks (RSNs) and baseline brain activities can be detected when the brain is at rest.These networks include the parts of the brain a*sociated with working memory. 'The RSNs have great potential for studying thalamic dysfunction in several clinical disorders including traumatic brain injury,' Dr.Ge said. Resting-state functional MRI (RS-fMRI) has rapidly emerged as a novel informative tool for investigating brain connectivity between regions that are functionally linked.RS-fMRI provides insight into functional activity and communication between brain regions, which play key roles in cognitive performance. 'The disruption of such functional properties is better characterized by RS-fMRI than by conventional diagnostic tools,' Dr.Ge said. Dr.Ge and colleagues used RS-fMRI to study the brain activity of 24 patients with MTBI and 17 healthy control patients.A normal pattern of thalamic RSNs with relatively symmetric and restrictive connectivity was demonstrated in the healthy control group.In the patients with MTBI, this pattern was disrupted, with significantly increased thalamic RSNs and decreased symmetry.These findings correlated with clinical symptoms and diminished neurocognitive functions in the patients with MTBI. 'The thalamic functional networks have multiple functions, including sensory information process and relay, consciousness, cognition, and sleep and wakefulness regulation,' Dr.Ge said.'The disruption of thalamic RSNs may result in a burning or aching sensation, accompanied by mood swings and sleep disorders, and can contribute to certain psychotic, affective, obsessive-compulsive, anxiety and impulse control disorders.These symptoms are commonly seen in MTBI patients with post-concussive syndrome.' Because the causes of post-concussive syndrome are poorly understood, there is currently no treatment.But, according to Dr.Ge, the results of this study have implications for a new therapeutic strategy, based on sound understanding of the underlying mechanisms of thalamocortical disruption and post-concussive syndrome. - Enviado mediante la barra Google" Health Solutions - Stress Relief
Steel buildings can be used for office, storage or living space.This material is used in construction of these places because it offers iron cladding or a strong structure.These buildings are made using steel, which is a form of alloy.It has between .2 and 2.1 percent in weight of carbon, this is relative to the grade.These structures were widely used during World War II and during the How to Prevent and Treat Asthma In Toddlers
IndiaВ is all set to produce over 4,400 more doctors every year. TheВ Medical Council of IndiaВ (MCI) has increased the number of seats for undergraduate medical education by 4,452 from this academic session (2011-12). Around 2,650 seats have been increased due to the opening of new colleges.And, the rest of seats (1,802) came about after the strength of the existing colleges was increased. India has around 335 medical colleges 185 and 150 in private public sector, respectively and will produce over 40,000 MBBS doctors every year. Even though the requirement is much higher, experts say the increased number of seats is a positive step forward. According to MCI’s governing body member Dr Purushottam Lal, the Council received 86 applications for permission to start medical colleges this year.Around 43 were disapproved on scrutiny, and 43 were inspected.Of those inspected, 22 weren’t cleared. Of the 21 new colleges cleared, 16 and five are in the private and public sector, respectively. TheВ MCIВ received 104 applications from registered colleges for further increase of seats.Around 43 were disapproved on scrutiny, and 61 were inspected.Of those inspected, only 33 (18 and 15 in private and public sector, respectively) fulfilled the criteria, and were given permission to ramp up their strength. «We’ve an additional 4,452 seats from this academic year itself for undergraduate medical education,» Dr Lal added. India faces a severe shortage of doctors. Earlier, a planning commission study had said India is short of six lakh doctors, 10 lakh nurses and 2 lakh dental surgeons because of which it has a dismal patient-doctor ratio. For every 10,000 Indians, there is one doctor.Another recent paper, «human resources for health in India», published in the British Medical JournalВ Lancet, said, there are eight healthcare workers, 3.8 allopathic doctors and 2.4 nurses per 10,000 population.When compared to other countries, this is about half the WHO benchmark of 25.4 workers per 10,000 people. MCI’s own note on «curricular reform in undergraduate medical education», available with TOI, says, the current doctor population ratio in India is 1:1700 when compared to a world average of 1.5:1000.The committee came reached a consensus that targeted doctor population ratio should be 1:1000 by 2031. [zeezal] Latest Photographs In Beautiful Model Bipasha Basu
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We went to visit CITTA today and were happy to see that she is doing well.She's a strong girl and is coping with what happened to her well except for the fact that she is still somewhat very frightened of strangers.The vet has scheduled her spaying as well as nose reconstruction surgery tomorrow and we are keeping our fingers crossed that all will be well.The vet also gave us some pics that were taken when Citta arrived at the clinic.It was painful to watch and act of such cruelty and brutality.We are posting the pics here so that you too can know what had happened to Citta. Inspirational Replay
nutrients.Established by the Institute of Medicine (National Academy of Sciences), the RDA is the average daily dietary intake level of a nutrient sufficient to meet the requirements of nearly all healthy individuals in a specific life stage and gender group.care practitioners recommend higher daily intakes of certain nutrients for people at risk for eye problems.In the following list, mg = milligram; mcg = microgram (1/1000 of a mg) and IU = International Unit.Omega-3 Fatty Acids* RDA: None.(For cardiovascular benefits, the American Heart Association recommends approximately 1,000 mg daily.) News: Xbox 360 Wireless Microphone
Last week we went on our annual Rushing River camping trip. It.Was.Awesome. We hiked. We swam. Seth swam so much.He made Neil swim out to the island over and over and over again and even jumped off the rock a few times.He loved it. В We had a lot of fun hanging out together.The weather was perfect- really hot. This wasn’t Oliver’s first time camping, but it was the first time he was old enough to enjoy it.And he really, really did. В Besides Oliver crying for two hours in the middle of the night the first night, the kids slept pretty good and so did we. We picked wild blueberries, saskatoons, and even raspberries, all right on our lovely campsite.We cooked corn on the cob, kebabs, and even made pizza.It was pretty delicious.We made s’mores too, of course, and the kids were pretty thrilled with the banana splits we cooked in the fire too. We were supposed to stay for three nights but we woke up to rain on our Wednesday morning and it just wouldn’t quit.We hung out in the tent for awhile. The kids fed each other Golden Grahams from each other’s mouths.Like baby birds. And there was wrestling of course. When it became clear that the rain wasn’t letting up, and our belongings inside the tent were getting wet, we decided we had to pack it in and call it a day.But don’t worry, we’ll be back next year!Because we love you Rushing River! kourtney kardashian baby
Heat precautions protect older people Today's THV Heart or blood tube problems or poorly employed sweat glands. - Heart, lung or kidney disease, as well as any illness that causes imperfection or fever. - advanced blood pressure or another conditions that require type A low-salt diet. - winning prescribed drugs such ...and moreВ » Link To Article The Ojai Cook Lemonaise Light 12 Oz.
visit now - budurl.com -Medical Researcher, Nutritionist, Health Consultant and Former Acne Sufferer Teaches You How To: Permanently Cure Your Acne Within 2 Months End The Breakouts & See Results In Less Than 7 Days!Eliminate Blackheads, Excessive Oiliness and Redness Remove Most Types of Scars and Acne Marks Look Better, Feel Better and Regain Your Self Esteem Improve The Quality Of Your Life Dramatically!Step-By-Step Fully Illustrated Guides Discover How He's Cured Himself From Severe Acne and Taught Thousands of People Worldwide to Get The Clearest Skin They Ever Had: Without Resorting To Drugs Without Creams Or Ointments Works on All Types of Acne: Vulgaris, Conglobata, Rosacea, Blackheads, Whiteheads and Cysts My Foundation Routine (For Acne Scars and Oily Skin)this is how i do my foundation.my skin is red, splotchy, and i have oily and acne prone skin.hope you all enjoyed...and aren't grossed out by my face without makeup...haha :) **beforehand, i cleanse and tone my face.i also use lush's vanishing cream moisturizer.it is extremely important to moisturize your face before you apply makeup, even if your skin is oily!Products I Used: Monistat Chafing Relief Powder Gel (primer) Sigma F50 Duo-Fiber Brush Maybelline Superstay Foundation in Sand Beige MAC Pro Longwear Concealer in NW20 (acne and scars) Benefit's Erase Paste Concealer in Fair 01 (undereye) ELF Powder Brush (Studio Line) MAC Mineralized Skin Finish Natural in Medium (a good drugstore alternative is Maybelline's Dream Matte Powder!) Urban Decay All Nighter Makeup Setting Spray and if ANY of you are wondering...probably not...my t-shirt is from hot topic and has bowser on it from mario...and it says like a boss...like from lonely island.mmm andy samberg...comment, rate, and subscribe if you like my videos!xoxo Major Break Via in Skin color Cancer Medication - The length of time Until it Gets to Market?
by Perfecto Insecto I've had acne for a few years now. As of right now, I'd say it's mild or maybe a little above mild but what makes it look terrible is the redness and oiliness of my skin! I was my face every morning and night with cetaphil (for normal to oily skin) and eat extremely healthy. My skin feels dry after i wash it but whenever I put moisturizer on my face it tends to make it really oily (it's an oil free moisturizer) and tends to break out even more! I'm about to go on a big date this Friday and I'm wanting to calm down my acne. Is there anything I can do in 4-5 days that will at least calm it down. Also!, any other tips that will help stop acne for good will be GREATLY appreciated! Answer by Luis i do not recomend puting moisturizer or any other type of cream on your face.it will make it go away for a day or 2 but it opens all your pores and many more will come out.so i juz recomend you get a towell and wash your face with just (water) nothing else water dosent open up your pores nd your acne will calm down.every 5 hours or so.hoped i helped Answer by Haloe You could go to the doctor's and get a prescription.They usually work pretty quickly.Or I've heard a lot of good things about Neutogena and ProActive if it'll take too long for the prescription to come in or your against it or something.I used to have really oily skin and I'm still battling with my achne, but if you're consistent with Cetaphil (which is what I use as well) once in the morning and once at night every day It should help.Oh and make sure you don't leave the Cetaphil on.You're only supposed to put it on and wash it off. Answer by Lady Rose Here are a few helpful, (and natural) hints for a better complexion for a lifetime: - avoid touching your face throughout the day (dirt, oils, & bacteria from your hands can clog the pores) - avoid any products with alcohol (internally or externally) Alcohol can actually burn & irritate sensitive or inflamed skin. - only use gentle, fragrance-free, oil-free, alcohol-free cleansers on your face - avoid any scented soaps and creams - have a daily facial cleansing routine (2x daily, morning & night.Do not scrub as this will irritate the skin) - try a dairy milk face-soak during a flare-up (as long as you're not allergic) The dairy milk helps to balance the natural PH of the skin - try using face lotion containing certified green algae or aloe vera gel (use lotions, not creams, as lotion is more gentle on the skin and absorbs more easily) - try using pure tea tree oil for pimples & blemishes.Apply with a q-tip to each individual pimple or blemish. - avoid extended periods in direct sun (use sunblock with 15 - 30 spf) - drink pure green tea, rose hip tea, and fennel tea (great antioxidants) - eat foods high in anti-oxidants; such as blueberries, cranberries, purple grapes, broccoli, etc.. - avoid spicy foods, greasy foods, and foods with a high refined-sugar content. - avoid all deep-fried food, oily food, foods high in fats (butter, margarine, salad dressings, etc..) Too much fat in the diet disrupts proper digestion. - many people find food with histamines aggravate the look of redness - avoid hot things.Hot showers & hot tubs, hot drinks (the heat will dilate blood vessels causing more redness) - try to get at least 7 - 8 hours of sleep each night (aids in the healing processes of the body) - try to cut down on stress (stress can magnify a multitude of conditions) Increase your omega 3 fatty acids by taking supplements such as Evening Primrose Oil, fish oils, etc And by eating walnuts, hazelnuts, or pecan nuts (if you're not allergic) Omega 3's aid in proper digestion and healthier skin. Ground Fennel seeds and Flax seeds, as well as Flax seed Oil supplements (omega 3's) also act as anti-inflammatories.(reduce redness) Try increasing your intake of vitamin D through supplements (1000 4000 IU/day) and B-complex to aid in healthier skin and maintaining a healthier immune system. Some people have had success using Turmeric orally or topically. (reduce redness and acne) Drink more water and avoid all alcohol & caffeine products (coffee, tea, pop, etc..) Alcohol & caffeine will actually dehydrate your skin.Water re-hydrates from the inside out. Neutrogena makes an excellent moisturizer, simply called "Moisture".It's oil-free, fragrance-free, alcohol-free and they have a type that is specifically made for sensitive skin.A little goes a long way, so the natural oils of your skin do not over-react. Remember, your skin needs to breathe to heal. I know there are other brands and products that may be similar.You need to find the one that works best with your skin and your body chemistry. Answer by Elizabeth Roe I have tried Pro Active and it didn't work for me.Tea Tree oil worked wonders for me, and right now I'm on the Dianette pill (obviously if you're a boy you cant take this pill) and it's worked wonders, my skin has never been better!I have oily skin too and tea tree oil does dry your skin out, but you only need the tiniest bit because it's very concentrated and strong.Once I used it too much, and it dried out my skin so much it was painful and felt like sunburn.I wouldn't use moisturiser, but Bio Oil because this is formulated to soak into the skin and not lube it up.It also reduces the appearance of scars from acne which has worked for me as well. ANYWAY.If your a girl, ask your doctor for Dianette.It's a contraceptive pill that doubles as an acne treatment, and if your a boy, go for tea tree oil.If you find your skin getting too dry, try Bio Oil or E45 Moisturiser (because it has no additives it is less likely to cause acne). Hope I helped!
Orignal From: How do i stop my acne and it's redness!!!? Recetas para salvar el SNS - DiarioMedico.com
I’m so in love with satchels.В So when my dear friend, O, showed me the Cambridge Satchel, I immediately fell in love with them and went crazy over the colors they offer! Don’t you just love the colors? * photo courtesy of whatiwore.tumblr.com After a few weeks of contemplating and stopping myself from buying, I finally gave up and gave in and I’m glad I did As if choosingВ whichВ color to get wasn’t hard enough, deciding on the right size was also tough!В :OВ It was pure dilemma!В But I’m happy with what I ended up getting, YELLOW in 11в
Functional parsing of driver mutations in the colo...[Cancer Res.2011] - PubMed result: "Cancer Res.2011 Jul 1;71(13):4359-65.Epub 2011 Apr 28.Eskiocak U, Kim SB, Ly P, Roig AI, Biglione S, Komurov K, Cornelius C, Wright WE, White MA, Shay JW.Landmark cancer genome resequencing efforts are leading to the identification of mutated genes in many types of cancer.The extreme diversity of mutations being detected presents significant challenges to subdivide causal from coincidental mutations to elucidate how disrupted regulatory networks drive cancer processes.Given that a common early perturbation in solid tumor initiation is bypa*s of matrix-dependent proliferation restraints, we sought to functionally interrogate colorectal cancer candidate genes (CAN-genes) to identify driver tumor suppressors.We have employed an isogenic human colonic epithelial cell (HCEC) model to identify suppressors of anchorage-independent growth by conducting a soft agar-based short hairpin RNA (shRNA) screen within the cohort of CAN-genes.Remarkably, depletion of 65 of the 151 CAN-genes tested collaborated with ectopic expression of K-RAS(V12) and/or TP53 knockdown to promote anchorage-independent proliferation of HCECs.In contrast, only 5 of 362 random shRNAs (1.4%) enhanced soft agar growth.We have identified additional members of an extensive gene network specifying matrix-dependent proliferation, by constructing an interaction map of these confirmed progression suppressors with approximately 700 mutated genes that were excluded from CAN-genes, and experimentally verifying soft agar growth enhancement in response to depletion of a subset of these genes.Collectively, this study revealed a profound diversity of nodes within a fundamental tumor suppressor network that are susceptible to perturbation leading to enhanced cell-autonomous anchorage-independent proliferative fitness.Tumor suppressor network fragility as a paradigm within this and other regulatory systems perturbed in cancer could, in large part, account for the heterogeneity of somatic mutations detected in tumors.Cancer Res; 71(13); 4359-65.В©2011 AACR. PMID: 21527559 [PubMed - in process] - Enviado mediante la barra Google" Recetas para salvar el SNS - DiarioMedico.com
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